Table 1.
F− recipient cell genotype | Effect of additional recipient mutations on the efficiency of recombination | |||
---|---|---|---|---|
None | priA300 | recF | priA300 recF | |
Wt | 1.00 | 0.82 ± 0.09 | 0.71 ± 0.02 | 0.70 ± 0.1 |
recG | 0.46 ± 0.09 | 0.90 ± 0.06 | 0.3a | 0.60 ± 0.07 |
ruvABC | 0.72 ± 0.18 | 0.22 ± 0.02 | 1.35 ± 0.28 | 0.97 ± 0.05 |
ruvABC recG | 0.0061 ± 0.0001 | 0.0077 ± 0.0026 | 0.044 ± 0.005 | 0.25 ± 0.03 |
ruvABC recG recO recB | 0.00095 ± 00018 |
Values are from crosses with Hfr donor strain GY2200, unless specified, and are means of at least two independent experiments, usually three or more, corrected for any reduction in the viability of the recipient cells. Error estimates are standard errors of the mean values. Uncorrected mean values, measures of recipient cell viability and of mating efficiency are documented in Table S2 along with the number of experiments conducted with each recipient
aData reproduced from (Ryder et al. 1994)