FIG 3.

Growth of UPP-Pase depletion strain under depletion/nondepletion conditions with different initial culture population size. Depletion strain (ganA::dCas9 SPβ::dCas9 ΔyodM uppP bcrC₁ bcrC₂) growing at logarithmic phase was diluted and reinoculated into liquid LB medium supplemented with either 1% (wt/vol) glucose or 2% (wt/vol) xylose. Growth was carried at 37°C with shaking. OD₆₀₀ was measured every 15 min. The starting culture with an OD₆₀₀ of ∼0.1 was designated dilution 1, and further twofold dilutions were performed until the smallest inoculum contained 1/8 the amount of the cells in dilution 1. Different initial inoculums affected the final OD of cells growing in LB xylose medium, while they had no significant effect on cells growing in LB glucose medium.