FIG 5.

Sensitivity of depletion strain against stresses under partial depletion conditions. Cells were grown on LB plates with chemicals added onto the filter paper disk. The sensitivity of the depletion strain was measured as the clear zone of inhibition around the paper disk. Chemicals used in this assay include bacitracin at 400 μg, ampicillin at 1 mg, penicillin at 1 mg, cefuroxime at 10 mg, vancomycin at 100 μg, fosfomycin at 1 mg, d-cycloserine at 100 μg, lysozyme at 100 μg, daptomycin at 100 μg, nisin at 1 mg, SDS at 10 μl of 10% solution, Triton X-100 at 10 μl of 25% solution, EDTA at 10 μl of 0.5 M (pH 8.0) solution, and novobiocin at 100 μg. The data are expressed as mean results ± standard errors (n = 3). Statistical analysis was performed using the t test with two samples assuming unequal variances. *, two-tailed P value of <0.05; **, two-tailed P value of <0.01.