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. 2016 Oct 7;198(21):2955–2964. doi: 10.1128/JB.00460-16

FIG 2.

FIG 2

(A) Schematic presentation of the principle of the two-plasmid system. A gene encoding A. brasilense sigma factor (Abr σ) is cloned downstream of the inducible PtacUV5 promoter in the pMMB206 vector. Upon induction by IPTG, the A. brasilense sigma factor is expressed from the PtacUV5 promoter. The A. brasilense sigma factor expressed in E. coli then brings E. coli core RNA polymerase binding to the A. brasilense promoter (Abr Pr) cloned upstream of the lacZ reporter in pCZ750 vector. If the A. brasilense target promoter is recognized by the A. brasilense sigma factor expressed in E. coli DH5α, β-galactosidase will be expressed, which can be assayed. (B) Effect of the expression of rpoH and rpoE paralogs of A. brasilense on the β-galactosidase activity of E. coli DH5α harboring crtE1::lacZ(pAPD3) or crtE2::lacZ(pAPD4) fusions. Vertical arrows indicate expression of the genes. The dashed baseline shows the β-galactosidase activity of E. coli DH5α harboring empty vector. Error bars show standard deviations from three replicates.