FIG 4.

(A) Effect of overexpression of the rpoH paralogs (indicated by upward arrows) on the carotenoid content of A. brasilense Sp7. Carotenoid content was compared by measuring absorption maxima at 485 nm of the methanolic extracts of the strains. The dashed baseline shows the carotenoid content in A. brasilense Sp7 harboring the empty vector. (B) Effect of overexpression of rpoE1(pAT5) or rpoE2(pNG6) on the β-galactosidase activity from lacZ fusions with the promoters of five rpoH paralogs of A. brasilense using the two-plasmid system in E. coli DH5α. pCZ750 and pMMB206 are the vectors used to construct lacZ fusions and express sigma factor genes, respectively. Error bars show standard deviations from three replicates.