FIG 8.

Binding of CsrA to crp and cyaA transcripts analyzed by EMSA. (A and C) Nucleotide sequences of the untranslated leader and initial coding region of crp (A) and cyaA (C) RNAs and the predicted sites (underlined) for CsrA binding. The first nucleotide of each sequence (•) is numbered with respect to the translation initiation site. Scores next to each predicted binding site (BS) are based on a position-weight-matrix analysis of CsrA binding sequences (27). The ATG and TTG initiation codons are bolded. (B and D) Binding and competition assays of CsrA with crp (B) and cyaA (D) transcripts. Positions of free (F) and bound (B) RNA are indicated.