Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 7;198(21):3016–3028. doi: 10.1128/JB.00530-16

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Szafran et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 2 — Contribution of the topA promoter to the total transcript level. (A) Nested RT-PCR using oligonucleotides complementary to the upstream region of the topA promoter (see the scheme at right). For the wild-type strain, the two bands (PCR1 and PCR2) correspond to the products of the p1 and p2 promoters (see the scheme at right). (B) RT-qPCR analysis of RNAs isolated from the wild-type strain and the p1 and p2 mutants. Oligonucleotides were designed to detect the overall topA transcript (p1 + p2) and p2-specific transcript (p2) levels (see the scheme at right).