FIG 3.

Residues near the C-terminal end of AmpG (AmpG region 4) modulated AmpG recycling efficiency. (A) Cartoon depiction of the AmpG replacement and AmpG chimera constructs expressed in N. gonorrhoeae (not drawn to scale). The AmpG replacement construct (top) was used as a base to generate the chimera constructs. Residues that differ between GC AmpG and MC AmpG are indicated in the order gonococcal residue, residue number, and meningococcal residue. Each chimera construct contained approximately one-quarter gonococcal ampG coding region and three-quarters meningococcal ampG coding region and contained a mixture of gonococcal and meningococcal residues. (B) PG fragment release profiles for N. gonorrhoeae strains expressing different versions of ampG.