FIG 5.

Increased recycling efficiency of gonococcal ampG mutants was not a consequence of increased AmpG protein levels. (A) AmpG was tagged with a C-terminal triple FLAG (FLAG3) epitope to determine AmpG levels made by N. gonorrhoeae via immunoblotting, comparing the levels of AmpG-FLAG3 expressed by different ampG mutant strains that have GC WT-like (EC512) or more efficient PG recycling (EC546, EC548, and EC550). (B) Quantification of band intensities from three independent experiments using LiCor Odyssey Fc.