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N. sicca and N. mucosa possess functional AmpG proteins. PG fragment release was examined for mutants carrying a kanamycin resistance cassette interrupting ampG in N. sicca (EC2004) (A) and N. mucosa (EC2003) (B) compared to WT N. sicca and N. mucosa. PG fragment release profiles for backcrossed mutants (EC2004BC and EC2003BC) are also shown. Quantification of the peaks was performed with data obtained from three independent experiments.
