TABLE 2.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| pIDN3 | Cloning plasmid containing GC/MC DNA uptake sequences | 30 |
| pHSS6 | Cloning plasmid, source of kanR | 64 |
| pEC026 | pIDN3 containing N. sicca/N. mucosa DNA uptake sequences | This study |
| pEC005 | ampGMCp-ampGGC WT cloned into pIDN3 | This study |
| pEC006 | ampGGCp-ampGMC WT cloned into pIDN3 | 23 |
| pEC007 | ampGGCp-ampGGC cloned into pIDN3 | This study |
| pEC008 | ampGMCp-ampGGC cloned into pIDN3 | This study |
| pEC013 | ampGGCp ampGMC cloned into pIDN3 | This study |
| pEC016 | ampGGCp ampGChimera 1 cloned into pIDN3 | This study |
| pEC017 | ampGGCp ampGChimera 2 cloned into pIDN3 | This study |
| pEC018 | mpGGCp ampGChimera 3 cloned into pIDN3 | This study |
| pEC019 | ampGGCp ampGChimera 4 cloned into pIDN3 | This study |
| pEC028 | ampGGC-FLAG3 cloned into pIDN3 | This study |
| pEC029 | ampGMC-FLAG3 cloned into pIDN3 | This study |
| pEC037 | ampGGCp-ampGGCM391L cloned into pIDN3 | This study |
| pEC038 | ampGGCp-ampGGCR398Q cloned into pIDN3 | This study |
| pEC039 | ampGGCp-ampGGCI402A cloned into pIDN3 | This study |
| pEC042 | ampGGCp-ampGGCM391L R398Q cloned into pIDN3 | This study |
| pEC043 | ampGGCp-ampGGCM391L I402A cloned into pIDN3 | This study |
| pEC054 | ampGGCp-ampGGCR398Q I402A cloned into pIDN3 | This study |
| pEC058 | ampGGCp-ampGGCM391L cloned into pIDN3 | This study |
| pEC063 | ampGN. sicca cloned into pEC026 | This study |
| pEC064 | ampGN. mucosa cloned into pIDN3 | This study |
| pEC067 | ampGN. mucosa::kan cloned into pIDN3 | This study |
| pEC070 | ampGN. mucosa::kan cloned into pEC026 | This study |
| pEC081 | ampGN. sicca::kan cloned into pEC026 | This study |
| pEC098 | ampGMC WT-FLAG3 cloned into pIDN3 | This study |
| pEC100 | ampGGCM391L-FLAG3 cloned into pIDN3 | This study |
| pEC102 | ampGGCI402A-FLAG3 cloned into pIDN3 | This study |
| pEC103 | ampGGCM391L R398Q I402A-FLAG3 cloned into pIDN3 | This study |