Fig. 4.
AβpE3-42 is more toxic than Aβ1-42 in Drosophila. Following treatment with RU486, Aβ1-42 and AβpE3-42 peptides were expressed at similar levels, quantified at 2 days and 21 days post-RU486 treatment (a) and (b) respectively. c A significant increase in the amount of insoluble to total Aβ protein levels in AβpE3-42 expressing flies in comparison to Aβ1-42 expressing flies was observed when quantified at 21 days post-RU486 treatment. Data are presented as means ± SEM and were analysed by Student’s t test, P < 0.01. d Expression of AβpE3-42 specifically in adult neurons shortens lifespan significantly relative to both Aβ1-42 and w1118 control. Survival curves are depicted and data were compared using the log-rank test. *P < 0.001 comparing elavGS/UAS-AβQ3-42 + RU flies to elavGS/+ and UAS-Aβ1-42 /+;elavGS/+ +RU flies