Fig. 6.

The C57SOD1^G93A mice have greater macrophagic activity than the 129SvSOD1^G93A in the sciatic nerve at disease onset. a–c Confocal micrographs show higher levels of CCL2 (green) in the sciatic nerve of C57SOD1^G93A than 129SvSOD1^G93A at disease onset and co-localization with the axonal marker Smi31 (red); scale bars, 50 μm. d Confocal micrographs showing the co-localization of the signal between CCL2 (green) and S100β (red) in the sciatic nerve of C57SOD1^G93A mice at disease onset; nuclei were stained with Dapi (blue); scale bar, 20 μm. e Representative western blot images of Iba1 and C3 on sciatic nerve extracts from the C57SOD1^G93A, 129SvSOD1^G93A mice, and Ntg littermates at disease onset. Densitometric analysis indicates a correlation between the levels of activation of f C3 and the marked upregulation of g Iba1 expression in the C57SOD1^G93A mice. The graph represents the mean ± SEM (n = 4 mice per group). ****P value <0.0001; **P value <0.01 (C57G93A vs 129SvG93A); °°°°P value <0.0001; °°°P value <0.005 (C57G93A vs 129SvNtg and or C57Ntg); ^§§ P value <0.01 (129SvG93A vs Ntg 129SvNtg and C57Ntg); ^#### P value <0.0001 (C57Ntg vs 129SvNtg and 129SvG93A) by two-way ANOVA with Tukey’s post-analysis. h Confocal micrographs showing higher levels of Iba1 (green) in the sciatic nerve of C57SOD1^G93A at disease onset; scale bar, 50 μm. i, j Confocal micrographs of longitudinal section of sciatic nerve of the C57SOD1^G93A mice showing the clear co-localization of signals between S100beta (red) and Iba1 (green); nuclei were stained with Dapi (blue); scale bars, (i) 20 μm; (j) 10 μm. k Confocal micrographs of longitudinal section of sciatic nerve of the C57SOD1 ^G93A mice showing the presence of Iba⁺ (green) S100β⁻ (red) cells; nuclei were stained with Dapi (blue); scale bar, 10 μm