Fig. 12.

Validation of putative Soly WRKY Group III genes by virus induced gene silencing (VIGS). a Cotyledon agroinfiltration of pTRV vectors was conducted in the resistant cultivar ‘Zheza-301’ at the cotyledon stage. After three weeks, leaves with pTRV1 and pTRV2-SolyWRKY showed normal phenotype. Leaves of ‘Zheza-301’ with pTRV1 and pTRV2-PDS showed bleached areas. b The expression level of two SolyWRKY Group III genes in the VIGS treated plants one week after agroinfiltration. A-Tubulin (Solyc04g077020.2) was used as an internal reference. ‘A’ represented the ‘Zheza-301’ treated with pTRV1 and pTRV2 (negative control), ‘VIGS’ represented the ‘Zheza-301’ with pTRV1 and pTRV2-SolyWRKY. c The accumulation of TYLCV DNA in the SolyWRKY Group III genes silenced ‘Zheza-301’ plants. ‘A’ represented the ‘Zheza-301’ treated with pTRV1 and pTRV2 (negative control), ‘VIGS’ represented the ‘Zheza-301’ with pTRV1 and pTRV2-SolyWRKY