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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: Nat Chem Biol. 2016 Aug 22;12(10):845–852. doi: 10.1038/nchembio.2153

Figure 4. Target binding affinity and cytotoxicity of stapled BIM BH3 libraries.

Figure 4

(a–b) BCL-XLΔC binding affinities as determined by fluorescence polarization assay of (a) staple scan and (b) point mutant FITC-BIM BH3 peptide libraries (peptide, 25 nM; protein 0.5 nM–1 μM). Binding isotherms are color coded based on relative binding potency (blue-green-red scale: blue, best binders; red, worst binders). Data are mean ± s.e.m. for experiments performed in technical quadruplicate and repeated twice. (c–d) The effect of stapled peptide treatment (0.3 μM–40 μM) on cell viability of BCL-XL-reconstituted p185+Arf−/−Mcl-1del B-ALL cells, as measured by CellTiter Glo assay at 24 hours. Data represent the mean of technical duplicates. The experiments were repeated three times with similar results.