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. Author manuscript; available in PMC: 2016 Oct 8.
Published in final edited form as: Biochemistry. 2016 Feb 1;55(6):970–979. doi: 10.1021/acs.biochem.5b01227

Figure 2.

Figure 2

UV–vis absorption spectra of (A) Fe- and Mn-TM0186 and (B) E169A and H173A Fe-TM0186. Proteins were at a concentration of 50 μM in 50 mM Tris, 50 mM NaCl, and 10 mM MgCl2 (pH 7.6). The weak absorption feature near 410 nm in the H173A and D289A Fe-TM0186 spectra is due to a very small amount of contaminating heme.