Table 2.
Qualitative c-Di-GMP PDE Activities of TM0186 and Its Variants
| TM0186 or varianta | form | excess metal addedb | c-di-GMP PDE productsc
|
|
|---|---|---|---|---|
| pGpG | GMP | |||
| Fe- | as-isolated | none | − | − |
| Mn | + | − | ||
| reduced | none | + | − | |
| Mn or Fe | + | + | ||
| Mn- | as-isolated | none | + | + |
| Mn or Fe | + | + | ||
| E169A Fe- | as-isolated | none | − | − |
| Mn | + | − | ||
| reduced | none | + | − | |
| Mn or Fe | + | − | ||
| E169A Mn- | as-isolated | none | + | − |
| Mn or Fe | + | − | ||
| H173A Fe- | as-isolated | none | − | − |
| Mn | + | − | ||
| reduced | none | − | − | |
| Fe | <+ | − | ||
| Mn | + | − | ||
| H173A Mn- | as-isolated | none | − | − |
| Fe | <+ | − | ||
| Mn | + | − | ||
| D289A Fe- | as-isolated | none | − | − |
| Mn | − | − | ||
| reduced | none | − | − | |
| Fe | − | − | ||
| Mn | − | − | ||
| D289A Mn- | as-isolated | none | − | − |
| Fe | − | − | ||
| Mn | − | − | ||
a
At 2 μM (monomer basis).
b
At 80 μM Fe2+ or 10 mM Mn2+.
c
Products observed 120 min after addition of c-di-GMP: −, no product; +, product; <+, low yield of product.