Table 2.

Sequential model fitting for all the responses (in terms of inhibition zone produced by bioactive metabolite)

Model parameter	S. aureus	B. subtilis	X. campestris	P. aeruginosa	C. albicans
Sequential model sum of squares—quadratic vs 2FI (suggested)
Sum of squares	85.83	59.22	237.14	94.56	26.82
df	5	5	5	5	5
Mean square	11.17	11.84	47.43	18.91	5.36
F value	2157.78	240.13	982.19	317.08	590.81
p value (Prob > F)	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
Lack of fit tests—quadratic (suggested)
Sum of squares	0.15	0.71	1.40	1.73	0.26
df	20	20	20	20	25
Mean square	7.535E−003	0.036	0.0070	0.086	1.08
F value	0.85	0.44	–	–	–
p value (Prob > F)	0.6410	0.9373	–	–	–
Model summary statistics—quadratic (suggested)
Std. Dev.	0.089	0.22	0.22	0.24	0.095
R 2	0.9975	0.9797	0.9944	0.9824	0.9925
Adjusted R 2	0.9957	0.9657	0.9906	0.9702	0.9873
Predicted R 2	0.9931	0.9503	0.9811	0.9551	0.9781

df degrees of freedom