Table 5.

ANOVA for Bacillus subtilis response surface quadratic model

Source	Sum of squares	df a	Mean square	F value	P value Prob > F
Model	68.97	20	3.45	69.91	<0.0001	Significant
A-time of incubation	0.49	1	0.49	10.01	0.0036
B-pH	0.70	1	0.70	14.10	0.0008
C-temperature	3.23	1	3.23	65.41	<0.0001
D-Sucrose	0.90	1	0.90	18.33	0.0002
E-Soya peptone	2.62	1	2.62	53.04	<0.0001
AB	0.0321	1	0.032	0.64	0.4301
AC	0.024	1	0.024	0.49	0.4895
AD	1.341E−003	1	1.341E−003	0.027	0.8702
AE	1.341E−003	1	1.341E−003	0.027	0.8702
BC	0.072	1	0.072	1.46	0.2366
BD	0.023	1	0.023	0.48	0.4960
BE	0.023	1	0.023	0.48	0.4960
CD	7.147E−003	1	7.417E−003	0.14	0.7062
CE	7.147E−003	1	7.147E−003	0.14	0.7062
DE	6.214E−003	1	6.214E−003	0.13	0.7252
A 2	0.74	1	0.74	14.92	0.0006
B	0.74	1	0.74	14.92	0.0006
C 2	2.21	1	2.21	44.82	<0.0001
D 2	0.052	1	0.052	1.06	0.3114
E 2	0.30	1	0.30	5.98	0.0207

^aDegrees of freedom