Table 1.
PCR primers used in this study.
| Gene | Primer | Nucleotide sequence (5′-3′) | Length of PCR product |
|---|---|---|---|
| cag A | cagA-F | GATAACAGGCAAGCTTTTGAGG | 349 |
| cagA-R | CTGCAAAAGATTGTTTGGCAGA | ||
|
| |||
| cag E | cagE-F | GTTACATCAAAAATAAAAGGAAGCG | 735 |
| cagE-R | CAATAATTTTGAAGAGTTTCAAAGC | ||
|
| |||
| cag T | cagT-F | TCTAAAAAGATTACGCTCATAGGCG | 490 |
| cagT-R | CTTTGGCTTGCATGTTCAAGTTGCC | ||
|
| |||
| cag M | cagM-F | ACAAATACAAAAAAGAAAAAGAGGC | 587 |
| cagM-R | ATTTTTCAACAAGTTAGAAAAAGCC | ||
|
| |||
| s1 and s2 | VA1-F | ATGGAAATACAACAAACACACC | 259 |
| VA1-R | CTGCTTGAATGCGCCAAACTTTATC | 286 | |
|
| |||
| s1a | SS1-F | GTCAGCATCACACCGCAAC | 190 |
|
| |||
| s1b | SS3-F | AGCGCCATACCGCAAGAG | 187 |
|
| |||
| s1c | S1C-F | CTTGCTTTAGTTGGGTTA | 213 |
|
| |||
| m1 | VA3-F | GGTCAAAATGCGGTCATGG | 290 |
| VA3-R | CCATTGGTACCTGTAGAAAC | ||
|
| |||
| m1T | m1T-F | GGTCAAAATGCGGTCATGG | 290 |
| m1T-R | CTCTTAGTGCCTAAAGAAACA | ||
|
| |||
| m2 | VA4-F | GGAGCCCCAGGAAACATTG | 352 |
| VA4-R | CATAACTAGCGCCTTGCAC | ||