Table 1.

List of DNA primer sequences designed for single-cell RT-PCR.

Target gene (product length)a		Outer primers	Inner primers	Genbank number
TRPV1 (273 bp, 203 bp)	Forward	TGATCATCTTCACCACGGCTG	AAGGCTTGCCCCCCTATAA	NM_001001445.1
	Reverse	CCTTGCGATGGCTGAAGTACA	CACCAGCATGAACAGTGACTGT
ChemR23 (481 bp, 273 bp)	Forward	GCCTCGCTAAAGCAACAAAC	TGGAGGAGTTCCACAAACAC	NM_027852.2
	Reverse	GCCAGCCTGTGCTATCTTAAT	GAGGCCCTTGCTTCAGAAT
NK1 (401 bp, 204 bp)	Forward	TCAGCCCTGGGAACCTATAA	CCCTGCTCCAAAGACCATTTA	NM_009313.5
	Reverse	GCTCCTCTCTAAGCAAGAACAG	CCACAGGTTGGAGGGAATG
GAPDH (367 bp, 313 bp)	Forward	AGCCTCGTCCCGTAGACAAAA	TGAAGGTCGGTGTGAACGAATT	XM_001473623.1
	Reverse	TTTTGGCTCCACCCCTTCA	GCTTTCTCCATGGTGGTGAAGA

(n1, n2)^a: n1 and n2 indicate the sizes of PCR products obtained from the outer and inner primers, respectively.