Figure 2.

Effect of folic acid on cytokines production and microglia activation in LPS-stimulated BV-2 cells. (a) Proinflammatory cytokine and microglia activation markers mRNA expression determined by real-time RT-PCR. BV-2 microglial cells were incubated for 6 h with LPS alone or after 1 h of pretreatment with different amounts of folates. (b) ELISA assay. Cells were treated with the indicated doses of folic acid for 1 h before LPS (1 μg/mL) treatment. Supernatants were collected at 24 h after LPS treatment and the amounts of IL1-β, IL-10, and TNF-α were measured. This assay was performed in triplicate and data are expressed as the mean ± SD of five independent experiments. ^∗ p < 0.05.