Fig. 2.

In vitro cellulose biosynthesis by reconstituted PttCesA8. (A) Time course of cellulose biosynthesis using UDP-[³H]-glucose as tracer. DPM, disintegrations per minute. (B and C) pH and temperature (B) and cation dependence (C) of PttCesA8’s catalytic activity. Synthesis reactions were performed in the presence of 20 mM of the indicated divalent cations. A catalytically inactive PttCesA8 mutant (CesA8*) and material obtained from a mock purification do not produce detectable glucan amounts. (D) Enzymatic degradation of the PttCesA8-synthesized glucan. All experiments were performed at least in triplicate; error bars represent the SDs from the means. AG, amyloglucosidase; AL, amylase; GH, glycoside hydrolase.