Table 1.

Distances across binding pocket in monomers of βI-tubulin and variants

Distance definition	Distance in complex	Average distance [Å] (% of snapshots with distance ≥ distance in complex)
		βI-Tubulin	T218A	T218A.S275A
23–215	18.39	20.1 ± 0.9 (97.3)	18.9 ± 1.4 (63.5)	19.3 ± 1.4 (76.0)
23–279	23.34	26.5 ± 1.3 (99.2)	23.1 ± 2.6 (51.5)	23.8 ± 3.4 (73.3)
23–360	11.34	12.4 ± 1.6 (73.3)	12.0 ± 1.4 (67.2)	13.2 ± 1.4 (91.2)
215–279	9.8	11.3 ± 1.0 (95.2)	12.8 ± 2.5 (85.3)	12.6 ± 1.5 (97.6)
215–360	16.43	17.6 ± 1.4 (80.3)	17.5 ± 1.3 (78.7)	17.8 ± 1.8 (79.5)
279–360	16.06	18.5 ± 1.7 (90.7)	14.7 ± 2.7 (36.3)	14.9 ± 3.4 (38.9)

Distance is measured between the Cα atoms of the two residues in the definition.