Table 3.
Mass isotopomer distribution of amino acids from cultures designed to explore the presence and activity of anaplerotic pathways in SYK-6
Amino acid | TBDMS fragment | 1-13C acetate | 1,2-13C acetate | |||||||
m0 | m1 | m2 | m0 | m1 | m2 | m3 | m4 | m5 | ||
Glutamate | [M-57]+ | 0.40 ± 0.03 | 0.37 ± 0.01 | 0.23 ± 0.02 | 0.34 ± 0.00 | 0.03 ± 0.01 | 0.11 ± 0.01 | 0.08 ± 0.00 | 0.13 ± 0.01 | 0.32 ± 0.01 |
[M-159]+ | 0.42 ± 0.01 | 0.57 ± 0.01 | 0.01 ± 0.01 | 0.35 ± 0.01 | 0.06 ± 0.01 | 0.12 ± 0.00 | 0.12 ± 0.00 | 0.35 ± 0.00 | ||
Aspartate | [M-57]+ | 0.51 ± 0.01 | 0.47 ± 0.00 | 0.02 ± 0.01 | 0.44 ± 0.03 | 0.07 ± 0.01 | 0.11 ± 0.00 | 0.13 ± 0.01 | 0.25 ± 0.03 | |
[M-159]+ | 0.74 ± 0.01 | 0.26 ± 0.01 | 0.43 ± 0.01 | 0.10 ± 0.01 | 0.17 ± 0.00 | 0.30 ± 0.01 | ||||
Alanine | [M-57]+ | 0.87 ± 0.00 | 0.13 ± 0.00 | 0.73 ± 0.00 | 0.06 ± 0.01 | 0.05 ± 0.00 | 0.16 ± 0.01 | |||
[M-159]+ | 0.95 ± 0.02 | 0.03 ± 0.00 | 0.02 ± 0.02 | 0.73 ± 0.00 | 0.08 ± 0.00 | 0.19 ± 0.00 |