Fig. 4.

Mfn2 levels do not affect components of the mitochondrial Ca²⁺ uptake machinery. (A and B) Equal amounts (40 μg) of protein were separated by SDS/PAGE and immunoblotted using the indicated antibodies from Mfn2^flx/flx MEFs infected with mtYFP andCRE-2A mtYFP (CRE) adenoviruses for 48 h. (C) Mean ± SEM of densitometric quantification of MCU, MICU1, and MICU2 protein levels in three independent experiments as in A. (D) RNAseq results for WT, cardiomyocyte-specific Mfn2 transgenic (Mfn2^tg), and cardiomyocyte-directed Mfn2 knockout (Mfn2^HKO) mouse hearts, expressed as read fragments per kilobase of exon per million reads mapped (FPKM). *P < 0.02 vs. WT (one-way ANOVA).