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Tethering is influenced by cell confluency. (A and B) equal amounts (40 μg) of protein from HUVEC cells (A) or WT and Mfn2−/− MEF (B) at indicated confluency were separated by SDS/PAGE and immunoblotted using the indicated antibodies. (C) Densitometric quantification of MCU protein levels in WT and Mfn2−/− MEFs at indicated confluency. Experiments were as in B. Data represent mean ± SEM of three independent experiments.
