Fig. 2.

Cell volume response of the wild-type cells at different downshock magnitudes shows slow volume recovery and an “overshoot.” We use descriptive statistics to present our datasets and plot average volume traces with SDs constructed from 609 (WT) and 480 [double mutant (DM)] traces as a function of time. A zoom-in to first 5 min of downshock response sampled every 0.375 s is given. In total, 64 (WT) and 66 (DM) cells were used for 103 mOsmol, 94 (WT) and 52 (DM) cells were used for 190 mOsmol, 66 (WT) and 54 (DM) for 390 mOsmol, 56 (WT) and 80 (DM) for 460 mOsmol, 90 (WT) and 68 (DM) for 790 mOsmol, 116 (WT) and 50 (DM) for 960 mOsmol, and 106 (WT) and 77 (DM) cells for the 1,130-mOsmol downshock. Volume expands in all conditions and increases with the shock magnitude. Slow recovery follows volume expansion. For the wild-type cells, volume drops below the initial value, increasingly so with the larger shocks.