Fig. 3.

Analysis of maximum and minimum volume and time. (A) Box plot of maximum volume, $V_{n\mathrm{,max}}$, and (B) the time at which maximum volume is reached, $T_{\max }$, as a function of shock magnitude. The wild type is shown in blue, and the double mutant, in red. The upper/lower whiskers indicate 1.5× the SD value. The upper/lower edges of the boxes indicate the third/first quartile. The black line indicates the median, and the yellow line, the average value. $V_{n\mathrm{,max}}$ increases with the shock magnitudes and saturates at and above 790 mOsmol. $T_{\max }$ is independent from the shock magnitude for the wild type (blue) and slightly smaller for the double mutant (red) for the two largest shock magnitudes. (C) $V_{n\mathrm{,min}}$ and (D) $T_{\min }$ plotted against the shock magnitude for wild type (blue) and double mutant (red). $V_{n\mathrm{,min}}$ in C is slightly below 1 for the wild type and decreases with the shock magnitude. $V_{n\mathrm{,min}}$ for the double mutant stays above 1 in all conditions. $T_{\min }$ increases with the shock magnitude for both wild-type and double-mutant cells.