Fig. 2.

HF diet alters differently AMP gene expression in duodenum, jejunum, and ileum. (A) Gene expression relative to AMP genes from HF-fed mice compared with SD-fed mice in duodenum, jejunum, and ileum (n = 10–12 per group). Results were normalized to those for the Gapdh gene expression. All values are means ± SEM. Means with asterisks are significantly different from values of SD-fed mice (**P < 0.01, ***P < 0.001). NS, not significant. (B) Representative photographs of immunofluorescence staining of Reg3γ protein (green) in unwashed duodenum, jejunum, and ileum sections of SD- and HF-fed mice. Nuclei stained with DAPI (blue). (Scale bars, 50 μm.)