Skip to main content
. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Curr Genet. 2016 Apr 11;62(4):725–730. doi: 10.1007/s00294-016-0599-x

Fig. 1.

Fig. 1

Tbf1, a Myb-like protein, interacts with Swi1 in S. pombe cells. a S. pombe cells were engineered to express Swi1-13Myc and/or Tbf1-5FLAG. Cell extracts were prepared, and Swi1-13Myc was immunoprecipitated with anti-Myc polyclonal antibodies. Whole-cell extracts and precipitated fractions were probed with the anti-Myc (9E10) or anti-FLAG (M2) antibodies, in order to detect Swi1-13Myc and Tbf1-5FLAG by Western blotting (WB). b Tfb1-FLAG was immunoprecipitated from cell extracts expressing Swi1-13Myc and/or Tbf1-5FLAG. Swi1-13Myc and Tbf1-5FLAG in whole-cell extracts and precipitated fractions were detected by Western blotting (WB). c Two-hybrid interactions between Swi1 and Tbf1 or Taz1. Gal4-AD-Swi1 was tested in the Y190 strain for interaction with Gal4-DBD-Tbf1 or Gal4-DBD-Taz1. Growth on the -His-Leu-Trp 3-aminotriazole (3AT) plate indicates protein interaction. The interaction of Gal4-AD-Swi3 and Gal4-DBD-Swi1 was used as a positive control for protein interaction