Fig. 2.

Swi1 is involved in telomere silencing. swi1⁺ and/or rap1⁺ genes were deleted from an S. pombe strain bearing reporter genes. Rap1 has been shown to be involved in telomere silencing (Fujita et al. 2012; Moser et al. 2009b). The his3⁺ and ura4⁺ genes were inserted at silent subtelomeric regions of chromosome 1 [TAS-tel(L)] and 2 [TAS-tel2(L)], respectively. Fivefold-serial dilutions of the indicated strains were spotted onto YES agar medium or minimal medium lacking histidine or uracil. The plates were then incubated for 2–3 days at 32 °C. Representative images of repeat experiments are shown. Growth on medium lacking nutrients indicates defects in silencing at subtelomeres