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. 2016 Oct 10;7:418. doi: 10.3389/fimmu.2016.00418

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Copyright © 2016 Wang, Chen, Banda, Holers, Wu, Moghimi and Simberg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modifications of hydroxyl groups of SPIO NWs and CL-NWs do not decrease complement C3 opsonization. (A) Alkylation and acylation of hydroxyls on SPIO NWs and CL-NWs; (B) modifications in (A) did not decrease or even increased C3 opsonization; (C) modification of CL-NW hydroxyls with acetyl, carboxymethyl, and sulfoethyl groups; (D) modifications in (C) did not significantly decrease C3 opsonization in human serum. Three different human sera were used in triplicates, and the results of a typical experiment are presented.