Skip to main content
. 2016 Oct 10;7:1614. doi: 10.3389/fmicb.2016.01614

FIGURE 2.

FIGURE 2

Secretion of IL-8 and hBD-3 in LS174T cells after treatment with S. Typhimurium SL1344, ΔyqiC, ΔyqiC′, ΔfliC, Salmonella flagellin, and IL-1β. LS174T cells were treated with S. Typhimurium SL1344 (MOI = 5), 100 ng/mL Salmonella flagellin, and 50 ng/mL IL-1β, according to the protocol in the bacterial invasion assay to generate output pool B. After 18-h treatment, the media from the final 15-h incubation in these wells were examined for quantification of IL-8 (A) and hBD-3 (B) secretion by using ELISA. Flagellin- and IL-1β-treated cells were the positive controls. The Student’s t-test was used for analyzing any significant differences in IL-8 and hBD-3 secretion levels between the S. Typhimurium SL1344-infected cells and the other groups (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001), and significant differences in IL-8 and hBD-3 secretion levels between negative control (LB-treated cells) and positive controls (††p < 0.01, †††p < 0.001).