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. 2016 Oct 10;7:1614. doi: 10.3389/fmicb.2016.01614

FIGURE 6.

FIGURE 6

Gene expression analysis of flagella, type-1 fimbriae, SPI-1, and SPI-2 in S. Typhimurium SL1344, ΔyqiC, and ΔyqiC′, and NADH dehydrogenase inhibition assays using qRT-PCR. (A) The mRNA expression levels of the type-1 fimbriae-related genes fimA and fimZ, the flagella-related genes flhD and fliZ, the SPI-1-related gene invA, and the SPI-2 gene sseB in S. Typhimurium ΔyqiC were compared with those of S. Typhimurium SL1344 and ΔyqiC′. (B) The NADH dehydrogenase inhibition assay revealed the effects of rotenone (Rot) and yqiC deletion on the regulation of the mRNA expression in flhD, fliZ, fimA, and fimZ, invA and sseB. The mRNA expression levels of the target genes are expressed as fold-change relative to the geometric means of their mRNA expression levels in S. Typhimurium SL1344. Any significant differences in the mRNA expression levels between S. Typhimurium SL1344 and other strains or conditions were analyzed using the Student’s t-test. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 were considered statistically significant.