Table 1.
Primers used for qRT-PCR.
| Primer (F: forward, R: reverse) | Sequence (5′–3′) | Product size (base pairs) | Description |
|---|---|---|---|
| fimA-F | CTAAATCCGCCGATCAAA | 193 | Type-1 fimbrial gene |
| fimA-R | GAGGAGACAGCCAGCAAA | 193 | Type-1 fimbrial gene |
| fimZ-F | GACGAACACCCTATTGTAAGA | 202 | Type-1 fimbrial gene |
| fimZ-R | GTTCCTGGATAGATTTGATTC | 202 | Type-1 fimbrial gene |
| flhD-F | GCATACATCCGAGTTGCTAA | 237 | Flagellar gene |
| flhD-R | TGAGTCAAACGGGTGATC | 237 | Flagellar gene |
| fliZ-F | GGAATATGTCGTGCGTTTA | 221 | Flagellar gene |
| fliZ-R | TATCAGAACTGGCGGTAAA | 221 | Flagellar gene |
| invF-F | TGAGAATGCTGGGAGAAGAC | 194 | SPI-1 gene |
| invF-R | AAAATGTGAAGGCGATGAGT | 194 | SPI-1 gene |
| sseB-F | AACGTGCCAGAAATACCCAG | 199 | SPI-2 gene |
| sseB-R | CCTTTATCCAGCTTCCCATG | 199 | SPI-2 gene |
| 16S-F | TTCCTCCAGATCTCTACGCA | 552 | Housekeeping gene 16S |
| 16S-R | GTGGCTAATACCGCATAACG | 552 | Housekeeping gene 16S |