Figure 2.

EP receptor expression in macrophages. Isolated RNA was converted to cDNA by reverse trancriptase (+), and as a control, this reaction step was also carried out in the absence of reverse transcriptase (−). Amplification of cDNA was performed using primers specific for each of the EP receptor subtypes and β‐actin. Expression profiles for three macrophage preparations (MAC1, MAC2 and MAC3) are shown. No mRNA for EP₁ receptors was detected in macrophages but, in separate experiments, the presence of EP₁ receptors could be readily demonstrated in several breast cancer cell lines, MDA‐MB‐468, MDA‐MB‐231 and ZR‐75‐1 (Kay et al., 2013). No mRNA for EP₃ receptors was detected, but in separate experiments, EP₃ receptors could be detected in the human mast cell line, LAD‐2 (Kay et al., 2013). These findings are representative of a total of five different macrophage preparations in excess of 95% purity. Lanes at either end of each gel represent a 100 bp ladder.