Figure 4.

Effects of EP receptor agonists on macrophages. Macrophages were pre‐incubated (30 min) with indomethacin (1 μM) and then with or without either (A) misoprostol, (B) L‐902,688, (C) butaprost or PGE₂ for 30 min before challenge with LPS (1 ng·mL⁻¹) for 22 h after which TNF‐α was measured in the supernatants. Results are expressed as the % inhibition of control cytokine release, which was 1379 ± 431 pg·mL⁻¹ of TNF‐α. Data shown are means ± SEM for five (A, B) or six (C) experiments.