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. 2016 Oct 10;6:34682. doi: 10.1038/srep34682

Figure 1. si-NLRP3 or si-ASC effect on omnipaque-induced cell apoptosis in HK-2 cells.

Figure 1

(a) Quantitative RT-PCR of NLRP3 and ASC. Cells were treated with 20 mg/ml mannitol (Manni.), 250 mgI/ml visipaque (Visip.), and 20 mgI/ml omnipaque (Omnip.) respectively for 72 h. (b) Quantification of cell viability by flow cytometry. HK-2 cells were treated with 20 mg/ml mannitol (Manni.), 250 mgI/ml visipaque (Visip.), and 20 mgI/ml omnipaque (Omnip.) respectively for 72 h. (c) Quantitative RT-PCR analysis of NLRP3 expression in si-NLRP3 cell and ASC expression in si-ASC cell, with si-NC (non-specific control) cell as control. (d) Quantification of cell apoptosis by flow cytometry. HK-2 cells transfected with si-NC or si-NLRP3 or si-ASC were treated with mannitol (Manni., 20 mg/ml), omnipaque (Omnip., 20–40 mgI/ml). si-ASC and si-NLRP3 dramatically attenuated omnipaque-induced cell apoptosis. Bars represent percentages of apoptotic cells according to the left data. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.