Skip to main content
. 2016 Oct 10;6:34682. doi: 10.1038/srep34682

Figure 3. NLRP3 deficiency effect on the expressions of apoptosis associated proteins in vivo responded to contrast media.

Figure 3

(a) qRT-PCR of NLRP3 and ASC in NLRP−/− mice following different treatments (n = 4 per group). 1. control; 2. model undergoing unilateral nephrectomy; 3. model + water deprivation for one day; 4. model + water deprivation for one day + furosemide injection; 5. model + water deprivation for one day + furosemide injection + contrast media. (b) Analysis of serum creatinine level in wild type (WT) mice and NLRP3−/− mice following different treatments (n = 4 per group). 1. control; 2. model undergoing unilateral nephrectomy; 3. model + water deprivation for one day; 4. model + water deprivation for one day + furosemide injection; 5. model + water deprivation for one day + furosemide injection + contrast media. (c) Western blot of NLRP3, ASC, procaspase-1,−3, −8 s, cleaved caspase-1,−3,−8 s, Bcl-2, and Bax in NLRP3−/− mice (n = 4 per group). 1, 4. control; 2, 5. model + water deprivation for one day + furosemide injection; 3, 6. model + water deprivation for one day + furosemide injection + contrast media. Data are shown as mean ± SD. *P < 0.05, ***P < 0.001.