Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 10;6:34605. doi: 10.1038/srep34605

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

MCD-db/db mice were treated with vehicle or IGF-I for 4 weeks using osmotic pump (n = 5 for each group). (a) Intraperitoneal glucose tolerance test and intraperitoneal insulin tolerance test. Data were compared by MANOVA test. (b) Visceral fat area visualized by computed tomography. Red areas show intraperitoneal fat. The osmotic infusion pump revealed a round-shaped and darkened figure. Quantitative analysis shows decreased visceral fat area in the IGF-I treatment group. (c) Histological analysis of the liver (hematoxylin-eosin staining, 100×) and Oil red-O staining (200×) and Triglyceride content in the liver. (d) Histological analysis of the liver (hematoxylin-eosin staining, 600×). The arrow heads denote ballooning necrosis of hepatocytes. Quantitative analysis of the number of hepatocytes showing ballooning necrosis. (e) The analysis of fibrosis by masson’s trichrome staining (400×). The arrow heads denote blue-colored pericellular fibrosis in the liver (upper panel 10×, lower panel 600×). Quantitative analysis of fibrotic area. Values are mean ± SEM. (f) Quantitative realtime PCR analysis of macrophage (Cd68 and F4/80), inflammatory (Tnfα), pro-inflammatory cytokines (Il-1β and Il-6), and fibrotic markers (Tgfβ, Procollagen 1a1, Collagen 3a1, and Collagen 4a1). Data were compared by Student’s t test. (g) Immunohistochemical analysis using Ly-6C (a marker for neutrophils) and CD68 antibodies (n = 5 for each groups). The arrow heads denote Ly-6C- and CD68-potitive (a marker for macrophages or Kupffer cells) cells. (f) Quantitative analysis of the number of Ly-6C and CD68-positive cells in the liver. Data were compared by Student’s t test.