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. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788

Figure 2. hVLCFAs are almost specific of SLs and 50 nM metazachlor alters the composition of VLCFAs in the pool of SLs.

Figure 2

In untreated Arabidopsis roots, the pool of FAs of glycerophospholipids (a) contains very few hFAs (d). Metazachlor significantly reduces the 24:0-containing FAs but does not alter the global composition of FAs of glycerophospholipids, which are mainly composed of C16- and C18-containing FAs. Arrowed brackets display the sum of<24 carbon atom-containing FAs and>24 carbon atom-containing FAs. Each histogram is further divided in hFAs (α-h) and non-h FAs (non-α-h). The ratio between<24- and>24-FAs is not altered in the glycerophospholipids pool. (b,c) Contrastingly to glycerophospholipids, in untreated roots, FAs of GlcCer (b) and GIPCs (c) contain a substantial amount of hFAs (d). Interestingly, metazachlor strongly reduces α-hydroxylated h24:1, h24:0 and h26:0 and increases α-hydroxylated h16:0, h20:0 and h22:0 FAs of GlcCer and GIPCs pools. Arrowed brackets display the sum of<24 carbon atom-containing FAs and>24 carbon atom-containing FAs. Each histogram is further divided in hFA α-h and non-hFA non-α-h. The ratio between<24- and>24-FAs is drastically inverted in both GlcCer and GIPCs pool. (e) Metazachlor neither alters the global quantity of FAs of the glycerophospholipids pool nor the global quantity of FAs of the GlcCer and GIPCs pools. Moreover, global quantities of individual sterols are not affected by metazachlor. Statistics were done by two-sided Wilcoxon's rank- sum test, *P-value<0.05, n=4 for each experiment, 4 biological replicates. Errors bars are s.d.