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. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788

Figure 7. Metazachlor accumulates PIN2 at SVs sites of TGN.

Figure 7

(a–o) Co-localization of endomembrane compartments labelled by PIN2–GFP (a,d,g,j,m) and either SYP61–CFP-labelled TGN-associated SVs (b), RAB-A5d-mCherry-labelled TGN-associated SVs (e), CLC-mOrange-labelled TGN-associated CCVs (h), MEMB12-mCherry-labelled Golgi apparatus (k) or SYP32-mCherry-labelled Golgi apparatus (n) on 50 nM metazachlor. (c,f,i,l,o) Merged pictures of corresponding pictures. (g) Quantification of co-localization events show a strong match of PIN2 with TGN-associated-SYP61/RAB-A5d-SVs, whereas weak co-localization is detected with MEMB12/SYP32-Golgi. (p) Co-localization values of SYP61/RAB-A5d with PIN2 and MEMB12/SYP32 with PIN2 are highly different. PIN2 co-localizes at medial level with TGN-associated-CLC-Clathrin vesicles but is significantly different from co-localization of PIN2 with SYP61/RAB-A5d. Statistics were done by two-sided Kruskal–Wallis rank sum test, XP-value>0.05, ***P-value<0.001, n=40 cells distributed over 10 roots for each experiment (3 biological replicates). All scale bars, 5 μm. Errors bars are s.e.m.