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. 2016 Sep 29;7:12993. doi: 10.1038/ncomms12993

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) B6.lpr.ICER/CREM^+/+ (n=7) and B6.lpr.ICER/CREM^−/− (n=8) mice were killed at 28 weeks of age. Kidney, spleen (n=7 and 8 mice, respectively) and cervical lymph nodes (n=6 per group) were dissected and weighted (*P<0.05, **P<0.01; mean±s.e.m.). (b) Periodic acid–Schiff (PAS)-stained kidney section and disease scores were shown (*P<0.05; mean±s.e.m.; n=5 per group). Scale bar, 50 μm. (c) C3 deposition in the kidney sections was assessed by direct immunofluorescence. Scale bar, 300 μm. Cumulative data are shown on the right (****P<0.0001; mean±s.e.m.; cumulative results of total 36 glomerulus from 5 B6.lpr.ICER/CREM^+/+ and total 40 glomerulus from 5 B6.lpr.ICER/CREM^−/− mice were assessed). (d) Percentage of double-negative T cells was evaluated by flow cytometry (8 B6.lpr.ICER/CREM^+/+ and 9 B6.lpr.ICER/CREM^−/−). Serum anti-dsDNA IgG (7 B6.lpr.ICER/CREM^+/+ and 6 B6.lpr.ICER/CREM^−/−) (e), urinary albumin to creatinine ratio (n=6 per group) (f) and serum IL-17A (7 B6.lpr.ICER/CREM^+/+ and 8 B6.lpr.ICER/CREM^−/−) (g) were quantified by ELISA (*P<0.05; mean±s.e.m.). (h) Kidney-infiltrated CD45⁺ cells, T cells and IL-17A-producing T cells were evaluated by flow cytometry (*P<0.05; mean±s.e.m.; 8 B6.lpr.ICER/CREM^+/+ and 9 B6.lpr.ICER/CREM^−/−). (i) Survival of indicated mice was monitored (8 B6.lpr.ICER/CREM^+/+ and 10 B6.lpr.ICER/CREM^−/−).