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. 2016 Sep 29;7:12979. doi: 10.1038/ncomms12979

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Copyright © 2016, The Author(s)

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(a) ESI-MS spectrum of the final ligation product of ubiquitin(1–75)-α-thioester with KAK^auxI. Calculated for KAK^Ub(aux)I, 9,081.1 Da. Observed KAK^UbI, 9,004.8±2.7 Da. (b) Time-course of N–O bond cleavage and KAK^UbI formation from the auxiliary-containing test substrate KAK^Ub(aux)I in a buffer consisting of 200 mM MPAA, 100 mM NaH₂PO₄ at pH 7.3 under the indicated conditions. Error bars represent the s.d. from three independent measurements.