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. 2016 Oct 10;6:34916. doi: 10.1038/srep34916

Figure 1. AU-FDS analysis of Htt-polyQ aggregation.

Figure 1

Flag pull downs were done as described in Materials and Methods. Galactose induction of Htt-polyQ expression was done at the times indicated, except in panel ‘a’ induction was done for 1.5 hr. A rotor speed of 25K was used in all experiments. The diploid was formed by mating W303-1A to W303-1B. In a particular panel the absolute c(S) values can be relatively compared. However, c(S) values between panels can not be absolutely reliably compared due to possible alterations in the fluorescence optics of the AU-FDS instrument over time and due to differences in total number of cells harvested for each separate experiment. However, in general as shown in Supplementary Figure 2, different strains harvested at relatively the same mid-log growth stage expressed relatively similar levels of Htt-polyQ, except for that containing the ssa1/ssa2 deletion as discussed in the text. (a) Htt-103Q, Htt-25Q, and no Htt. (b) Htt-25Q; (c) Htt-103Q; (d) Htt-47Q; and (e). Htt-103QP; and f. Diploid containing Htt-103Q.