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. 2016 Oct 10;6:34860. doi: 10.1038/srep34860

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Conservation of the murine and human Dies1 seed region for miR-125a-5p. Asterisks stand for conserved nucleotides, dashes for complementary regions. (b) Dies1 and miR-125a-5p expression in gastric cancer cell lines in comparison with normal stomach mucosa (in log scale). Also included is a summary of the Dies1 promoter methylation status (same legend as in Fig. 1 applies), for comparison and correlation purposes. Asterisks stand for significantly distinct comparisons (p < 5.00E-02). (c) RNA expression analysis confirming miR-125a-5p inhibition (from 0,016x to 0,001x vs. negative control) and concomitance increased expression of Dies1 (from 1,58x to 1,94x vs. negative control), ID2 (from 0,87x to 2,19x vs. negative control) and ID3 (from 0,92x to 1,87x vs. negative control. (d) Representation of the RNA expression analysis results correlating miR-125a-5p expression with Dies1 expression for each biological replicate performed.