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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Biochemistry. 2016 Aug 10;55(33):4636–4641. doi: 10.1021/acs.biochem.6b00565

Figure 2.

Figure 2

Identification of CEL as a FAHFA hydrolase. (a) 9-PAHSA hydrolysis activity for membrane lysates from mock-transfected and CEL-transfected HEK293T cells, and CEL-transfected membrane lysates treated with WWL92 (20 μM and 40 μM). Data represents mean ± s.d for two replicates. ***p < 0.001 by ANOVA. (b) 9-PAHSA hydrolysis and gel-based ABPP (inset) for WT pancreatic membrane lysates treated with DMSO or WWL92 (10 μM). (c) 9-PAHSA hydrolysis and western blot analysis (inset) of of CEL+/+ and CEL−/− pancreatic membrane lysates. Data represents mean ± s.e.m. for three biological replicates. ***p < 0.001 by Student’s t-test. Full images of gel and western blots are provided in Supplementary Figure 3.