Skip to main content
. Author manuscript; available in PMC: 2016 Oct 10.
Published in final edited form as: Biochem J. 2014 Aug 15;462(1):143–152. doi: 10.1042/BJ20140494

Figure 3. CLK1 N-Terminus interacts with the SRSF1 RS domain.

Figure 3

A) GST-tagged Constructs of SRSF1. B) Pull-down assays using His-N and GST-tagged constructs on g-agarose resin. An asterisk denotes an impurity in the His-N preparation and a double asterisk denotes an impurity in the GST-SR(ΔRS) preparation. C) Pull-down assays using GST-N and His-tagged kinases and g-agarose resin. Kinases are probed using an anti-His antibody. I = Input, PD = pull down. Vertical dashed line indicates where lane from the same gel is spliced.