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. Author manuscript; available in PMC: 2016 Oct 10.
Published in final edited form as: Biochem J. 2014 Aug 15;462(1):143–152. doi: 10.1042/BJ20140494

Figure 5. Phosphorylation-dependent binding of SRSF1 to the Ron ESE.

Figure 5

A) CLK1 phosphorylation induces cooperative binding of SRSF1 to the Ron ESE. Fraction bound is plotted against the total concentration of unphosphorylated (●) and CLK1-phosphorylated SRSF1 (△). The values of N and K0.5 are 1.2 ± 0.1 and 66 ± 2 nM for unphosphorylated SRSF1 and 2.0 ± 0.10 and 65 ± 2 nM for CLK1-phosphorylated SRSF1. B) CLK1(ΔN) phosphorylation does not induce cooperative binding of SRSF1 to the Ron ESE. Fraction bound is plotted against the total concentration of unphosphorylated (from panel A) and CLK1(ΔN)-phosphorylated SRSF1 (□). The values of N and K0.5 are 1.2 ± 0.1 and 96 ± 5 nM for CLK1(ΔN)-phosphorylated SRSF1.